Differentiation of mononuclear precursors into osteoclasts on the surface of Si-substituted hydroxyapatite

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Botelho, CM (Autor) Outra; Brooks, RA (Autor) Outra; Spence, G (Autor) Outra; McFarlane, I (Autor) Outra; Lopes, MA (Autor) FEUP; Best, SM (Autor) Outra; Santos, JD (Autor) FEUP; Rushton, N (Autor) Outra; Bonfield, W (Autor) Outra

Revista

Título: Journal of Biomedical Materials Research - Part A

Vol. 78A Nº 2

Páginas: 709-720

ISSN: 1549-3296

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 52 Citações

ISI Web of Science

Scopus - 61 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental

Outras Informações

ID Authenticus: P-004-H78

DOI: 10.1002/jbm.a.30726

Abstract (EN): In healthy bone, resorption and synthesis are in perfect coordination. In previous studies we demonstrated that the incorporation of silicon into the hydroxyapatite (HA) lattice enhances the proliferation and differentiation of human osteoblasts. Therefore, the aim of this study was to demonstrate the effect of silicon-substituted HA (0.8 and 1.5 wt % Si-HA) on the differentiation of mononuclear cells into osteoclasts, using two different starting cultures, peripheral blood mononuclear cells (PBMC) and monocytes expressing the CD14 antigen (CD14(+)). Through this study, it was possible to demonstrate that Si-HA allows the differentiation of mononuclear cells into mature osteoclasts, independent of the starting culture, PBMC or CD14(+). Most of the cells on the surface of the materials expressed osteoclastic markers: actin rings, several nuclei, positivity for tartrate-resistant acid phosphatase (TRAP), and vitronectin receptor. In the presence of osteoclasts, a higher release of calcium and phosphate into the medium from the 1.5 wt % Si-HA substrate was detected when compared to the HA substrate; therefore, these results indicate higher osteoclastic resorptive activity on the 1.5 wt % Si-HA surface. Si-HA can be resorbed by cellular mechanisms and have a stimulatory effect on osteoclasts, although the underlying mechanism is still poorly understood. (c) 2006 Wiley Periodicals, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12