Specific glutathione-S-transferases ensure an efficient detoxification of diclofenac in Solanum lycopersicum L. plants

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Sousa, B (Autor) Outra; Lopes, J (Autor) Outra; Leal, A (Autor) Outra; Martins, M (Autor) Outra; Soares, C (Autor) Outra; Manuel Azenha (Autor) FCUP; Fernanda Fidalgo (Autor) FCUP; Jorge Teixeira (Autor) FCUP

Revista

Título: Plant Physiology and Biochemistry

Vol. 168

Páginas: 263-271

ISSN: 0981-9428

Editora: Elsevier

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Outras Informações

ID Authenticus: P-00V-JFT

DOI: 10.1016/j.plaphy.2021.10.019

Abstract (EN): Diclofenac (DCF) is a very common pharmaceutical that, due to its high use and low removal rate, is considered a prominent contaminant in surface and groundwater worldwide. In this study, Solanum lycopersicum L. cv. MicroTom (tomato) was used to disclose the role of glutathione (GSH)-related enzymes, as GSH conjugation with DCF is a well reported detoxification mechanism in mammals and some plant species. To achieve this, S. lycopersicum plants were exposed to 0.5 and 5 mg L-1 of DCF for 5 weeks under a semi-hydroponic experiment. The results here obtained point towards an efficient DCF detoxification mechanism that prevents DCF bioaccumulation in fruits, minimizing any concerns for human health. Although a systemic response seems to be present in response to DCF, the current data also shows that its detoxification is mostly a root-specific process. Furthermore, it appears that GSH-mediated DCF detoxification is the main mechanism activated, as glutathione-S-transferase (GST) activity was greatly enhanced in roots of tomato plants treated with 5 mgL(-1) DCF, accompanied by increased glutathione reductase activity, responsible for GSH regeneration. By applying a targeted gene expression analysis, we provide evidence, for the first time, that SlGSTF4 and SlGSTF5 genes, coding for GSTs from phi class, were the main players driving the conjugation of this contaminant. In this sense, and even though tomato plants appear to be somewhat tolerant to DCF exposure, research on GST activity can prove to be instrumental in remediating DCF-contaminated environments and improving plant growth under such conditions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9