Molecular characterization, 3D modelling and expression analysis of sea bass (Dicentrarchus labrax L.) interleukin-10

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Pinto, RD (Autor) Outra; Nascimento, DS (Autor) Outra; Reis, MIR (Autor) Outra; do Vale, A (Autor) Outra; dos Santos, NMS (Autor) Outra

Revista

Título: Molecular Immunology

Vol. 44

Páginas: 2066-2075

ISSN: 0161-5890

Editora: Elsevier

Indexação

Scopus - 58 Citações

Outras Informações

ID Authenticus: P-00F-HHV

DOI: 10.1016/j.molimm.2006.09.008

Abstract (EN): Interleukin-10 (IL-10) is a pleiotropic cytokine generally known for its relevance in the resolution of inflammation, but that also has immunostimulatory properties. Here is described the isolation and characterization of the sea bass IL-10 (sbIL-10) cDNA and gene. The sbIL-10 gene encodes a 187 amino acid protein and comprises a five exon-four intron structure as other known IL-10 genes. Important structural residues are maintained in the sbIL-10 protein, including the four cysteines responsible for the two intra-chain disulfide bridges reported for human IL-10. The 3D structure of sbIL-10 was predicted. This first homology model of a fish IL-10 reveals a high degree of compatibility between the dimeric quaternary architectures of sbIL-10 and its mammalian counterparts. The phylogenetic analysis clusters sbIL-10 with other IL-10s, apart from IL-10-related molecules. The involvement of IL-10 in sea bass immune responses was demonstrated by investigating the expression profiles of IL-1ß and IL-10 in the head-kidney and spleen following intraperitoneal injection of UV-killed Photobacterium damselae ssp. piscicida. Furthermore, involvement of IL-10 in the resolution of inflammation is for the first time suggested in fish, due to the delayed maximal mRNA levels of sbIL-10 compared to those of the pro-inflammatory IL-1ß.

Idioma: Inglês

Tipo (Avaliação Docente): Científica