Regucalcin is expressed in rat mammary gland and prostate and down-regulated by 17 beta-estradiol

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Maia, CJB (Autor) Outra; Santos, CR (Autor) Outra; Fernando Schmitt (Autor) FMUP; Socorro, S (Autor) Outra

Revista

Título: Molecular and Cellular Biochemistry

Vol. 311

Páginas: 81-86

ISSN: 0300-8177

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 29 Citações

Outras Informações

ID Authenticus: P-004-0NC

DOI: 10.1007/s11010-007-9697-x

Abstract (EN): Regucalcin is involved in maintenance of calcium homeostasis due to the activation of Ca2+ pumping enzymes in the plasma membrane. It has a suppressive effect in cell proliferation, DNA and RNA synthesis, and may be associated with the abnormal cell division on tumor tissues. On the other hand both estrogens and Ca2+ are implicated in breast and prostate cancer but there are no studies focused on the expression of regucalcin in rat mammary gland or prostate. Furthermore, it is known that the expression of regucalcin in rat liver and kidney is regulated by 17 beta-estradiol (E-2). The aim of this study is to analyze if regucalcin is expressed in rat mammary gland and prostate and if it is regulated by E-2 in these tissues. We demonstrated for the first time that regucalcin mRNA and protein are present in rat mammary gland and prostate by in situ hybridization and immunohistochemistry, respectively. Furthermore, we show by Real-time PCR that E-2 down-regulates regucalcin expression in rat mammary gland and prostate.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6