Characterization of oligoadenylate synthetase-1 expression in rat mammary gland and prostate: effects of 17 beta-estradiol on the regulation of OAS1g in both tissues

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Maia, CJB (Autor) Outra; Socorro, S (Autor) Outra; Fernando Schmitt (Autor) FMUP; Santos, CRA (Autor) Outra

Revista

Título: Molecular and Cellular Biochemistry

Vol. 314

Páginas: 113-121

ISSN: 0300-8177

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 5 Citações

Outras Informações

ID Authenticus: P-003-Y98

DOI: 10.1007/s11010-008-9771-z

Abstract (EN): OAS1 belongs to a protein family of interferon-induced enzymes characterized by their ability to catalyze the synthesis of 2'-5'-linked oligomers of adenosine from ATP (2-5A). 2-5A bind to the latent Ribonuclease L (RNase L), which subsequently dimerizes into the active form, acquiring the capacity of cleaving cellular and viral mRNA. Several studies indicate that OAS1 is an important inducer of apoptosis in human cancer cells and that it may be regulated by 17 beta-estradiol (E(2)). The aim of this study was to characterize OAS1 gene expression in rat mammary gland and prostate, and to analyze its regulation by E(2) in both tissues. It is demonstrated that OAS1g is the most abundant OAS1 gene expressed in both tissues, and that OAS1 protein is present in the nucleus of rat mammary gland and prostate epithelial cells. In addition, it is shown by Real Time PCR that OAS1g is up-regulated by E(2) in rat mammary gland, but down-regulated in prostate, suggesting that the OAS1g gene may be related to estrogen dependent pathways in rat mammary gland and prostate physiology.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9