Reproductive hormones affect follicular cells and ooplasm of Stage I and II oocytes in zebrafish

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Sousa, ML (Autor) Outra; Silva, A (Autor) Outra; Malhao, F (Autor) Outra; rocha, mj (Autor) ICBAS; rocha, e (Autor) ICBAS; Urbatzka, R (Autor) Outra

Revista

Título: Reproduction, Fertility and Development

Vol. 28

Páginas: 1945-1952

ISSN: 1031-3613

Editora: CSIRO Publishing

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00H-MJH

DOI: 10.1071/rd15100

Abstract (EN): The basic pathway of oocyte development and its regulation is evolutionarily conserved among vertebrates; however, little is known about the role of hormones at the first stages (Stages I and II) of follicle development in fish. In the present study, zebrafish follicles at Stages I and II were exposed in vitro to the reproductive hormones 17-oestradiol (E-2), 11-ketotestosterone (11KT), 17,20-dihydroxy-4-pregnen-3-one (DHP) and to the secondary messenger dibutyryl cyclic adenosine monophosphate (db-cAMP) at a concentration of 1 mu M for a 48-h period. Morphological alterations of the ooplasm were assessed by transmission electron microscopy and of the granulosa cell layer by quantitative stereology. Expression of mRNA was analysed for cell-cycle genes (cyclin B and E) and resident proteins of the endoplasmic reticulum (calnexin and 78-kDa glucose-regulated protein (grp78/bip)). E-2 and db-cAMP stimulated the presence of endoplasmic reticulum in the ooplasm and calnexin mRNA increased in the db-cAMP treatment, but also in response to 11KT and DHP. 11KT, DHP and db-cAMP inhibited the progression of the cell cycle in the granulosa-theca cell layer, indicated by a reduction of the nucleus volume-weighted size of granulosa cells and of increased cyclin E mRNA expression. Reproductive hormones had different effects on the ooplasm and the granulosa-theca cell layer of zebrafish follicles, predominantly at Stage II.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8