An Improved LC-MS/MS Method for the Analysis of Thirteen Cytostatics on Workplace Surfaces

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Maria Francisca Cunha (Autor) FEUP; Sara Ramos (Autor) Outra; Adrián M. T. Silva (Autor) FEUP; Pedro Norton (Autor) Outra; Arminda Alves (Autor) FEUP; Mónica Santos (Autor) FEUP

Revista

Título: Pharmaceuticals

Vol. 14 Nº 8

Páginas: 1-17

ISSN: 1424-8247

Editora: MDPI

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 4 Citações

Projetos Associados

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LA LSRE-LCM - Financiamento Plurianual 2020-2023 para a Unidade LA LSRE-LCM Laboratório de Processos de Separação e Reacção - Laboratório de Catálise e Materiais

Outras Informações

ID Authenticus: P-00V-C9H

DOI: 10.3390/ph14080754

Abstract (EN): Cytostatics are drugs used in cancer treatment, which pose serious risks to healthcare workers. Dermal absorption via surface contamination is the key exposure route; thus, rapid, reliable, and validated analytical methods for multicomponent detection are crucial to identify the exposure risk. A surface-wipe-sampling technique compatible with hospitals' safety requirements (gauze, 1 mL isopropanol) and a fast and simple extraction method (1 mL acetonitrile, 20 min ultrasonic bath, evaporation, reconstitution in 200 mu L acetonitrile), coupled with liquid chromatography-tandem mass spectrometry analysis, were developed. It allowed identification and quantification of 13 cytostatics on surfaces: cyclophosphamide, doxorubicin, etoposide, ifosfamide, paclitaxel, bicalutamide, capecitabine, cyproterone, flutamide, imatinib, megestrol, mycophenolate mofetil, prednisone. Good linearity, sensitivity, and precision were achieved (R-2 > 0.997, IDLs < 4.0 pg/cm(2), average CV 16%, respectively). Accuracy for four model surfaces (melamine-coated wood, phenolic compact, steel 304, steel 316) was acceptable (80 +/- 12%), except for capecitabine and doxorubicin. Global uncertainty is below 35% for concentrations above 100 pg/cm(2) (except for capecitabine and doxorubicin)-a guidance value for relevant contamination. Method application in a Portuguese university hospital (28 samples) identified the presence of seven cytostatics, at concentrations below 100 pg/cm(2), except for three samples. The widespread presence of cyclophosphamide evinces the necessity to review implemented procedures.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17