Can the adrenergic system be implicated in the pathophysiology of bladder pain syndrome/interstitial cystitis? A clinical and experimental study

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Charrua A. (Autor) Outra; Pinto, R (Autor) FMUP; Taylor, A (Autor) Outra; Canelas, A (Autor) Outra; Ribeiro Da Silva, A (Autor) Outra; Cruz C.D. (Autor) FMUP; Birder, LA (Autor) Outra; Francisco Cruz (Autor) FMUP

Revista

Título: Neurourology and Urodynamics

Vol. 34

Páginas: 489-496

ISSN: 0733-2467

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 29 Citações

Outras Informações

ID Authenticus: P-00G-32J

DOI: 10.1002/nau.22542

Abstract (EN): AimsTo evaluate sympathetic system activity in bladder pain syndrome/interstitial cystitis (BPS/IC) patients and to investigate if chronic adrenergic stimulation in intact rats induces BPS/IC-like bladder modifications. MethodsClinical studyIn BPS/IC patients and aged and body mass index matched volunteers TILT test was undertaken and catecholamines were measured in plasma and 24hr urine samples. Experimental studyPhenylephrine was injected subcutaneously (14 days) to female Wistar rats. Pain behavior, spinal Fos expression, urinary spotting, number of fecal pellets expelled, frequency of reflex bladder contractions, and urothelial height were analyzed. Urothelium permeability was investigated by trypan blue staining. Immunoreactivity against caspase 3 and bax were studied in the urothelium and against alpha-1-adrenoreceptor and TRPV1 in suburothelial nerves. Mast cell number was determined in the sub-urothelium. In rats with lipopolysaccharide-induced cystitis, urinary catecholamines, and Vesicular Monoamine Transporter 2 (VMAT2) expression in bladder nerves were analyzed. ResultsThe TILT test showed an increase of sympathetic activity. Noradrenaline levels in blood at resting conditions and in 24-hr urine samples were higher in BPS/IC patients. Phenylephrine administration increased visceral pain, spinal Fos expression, bladder reflex activity, urinary spotting and the number of expelled fecal pellets. The mucosa showed urothelial thinning and increased immunoreactivity for caspase 3 and bax. Trypan blue staining was only observed in phenylephrine treated animals. Suburothelial nerves co-expressed alpha1 and TRPV1. Mastocytosis was present in the suburothelium. Cystitis increased sympathetic nerve density and urinary noradrenaline levels. ConclusionsExcessive adrenergic stimulation of the bladder may contribute to the pathophysiological mechanisms of BPS/IC. Neurourol. Urodynam. 34:489-496, 2015. (c) 2013 Wiley Periodicals, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8