Association and Phase Behavior of Cholic Acid-Modified Dextran and Phosphatidylcholine Liposomes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Guangyue Bai (Autor) Outra; Marieta Nichifor (Autor) Outra; Margarida Bastos (Autor) FCUP

Revista

Título: Journal Of Physical Chemistry Letters

Vol. 1 Nº 6

Páginas: 932-936

ISSN: 1948-7185

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 15 Citações

Classificação Científica

CORDIS: Ciências Físicas > Química > Química aplicada ; Ciências Físicas > Química > Química física

FOS: Ciências da engenharia e tecnologias > Engenharia dos materiais

Outras Informações

ID Authenticus: P-003-88M

DOI: 10.1021/jz100087k

Abstract (EN): The interaction between liposomes (1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)) and a hydrophobically modified water-soluble polymer (HMP; a bile acid-modified dextran)has been investigated by isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC), combined with turbidity measurement and cryogenic scanning electron microscopy (cryo-SEM). The thermodynamic information on the association (enthalpy of interaction, enthalpy of transition of mixed vesicles to mixed micelle-like aggregates) was obtained from lit. Further, the phase behavior for the system could be derived from the lit measurements, and be confirmed by turbidity and cryo-SEM. The effect of cholic acid (CA) side groups on the ordered arrangement of DMPC bilayers Was studied by DSC, by following the changes they induce in the gel-to-liquid crystalline liposome phase transition. The DSC results were in excellent agreement. with the interpretation proposed for the ITC results. The morphology of the aggregates; as characterized by cryo-SEM, is in line with the proposed aggregate morphologies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5