Macrophage Metabolomics Reveals Differential Metabolic Responses to Subtoxic Levels of Silver Nanoparticles and Ionic Silver

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Carrola, J (Autor) Outra; Bastos, V (Autor) Outra; Daniel da Silva, AL (Autor) Outra; Gil, AM (Autor) Outra; Santos, C (Autor) FCUP; Oliveira, H (Autor) Outra; Duarte, IF (Autor) Outra

Revista

Título: European Journal of Inorganic Chemistry

Vol. 2020

Páginas: 1867-1876

ISSN: 1434-1948

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00S-5YA

DOI: 10.1002/ejic.202000095

Abstract (EN): This study employed NMR metabolomics to characterize macrophage responses to subtoxic concentrations of silver nanoparticles (AgNPs ca. 30 nm) and ionic silver (Ag+), with a view to further elucidate their immunomodulatory activity at the cell metabolism level. Exposure to AgNPs caused RAW 264.7 macrophages to decrease intracellular glucose utilization, possibly due to interference with glycolytic enzymes, and to reprogram the TCA cycle towards anaplerotic fueling and production of anti-inflammatory metabolites (e.g. itaconate and creatine). Moreover, AgNPs-exposed cells were able to control the levels of reactive oxygen/nitrogen species (ROS/RNS), likely through upregulation of glutathione synthesis. On the other hand, macrophages exposed to Ag+ at equivalent subtoxic concentrations showed reduced metabolic activity, lower ability to counterbalance ROS/RNS and alterations in membrane-related lipids. Overall, the metabolomics approach hereby employed provided novel insights into the differential effects of AgNPs and Ag+, which help explain the lower toxic potential of nanosilver than silver ions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10