Superoxide dismutase expression in human cumulus oophorus cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Matos, L (Autor) FCNAUP; Stevenson, D (Autor) Outra; Gomes, F (Autor) Outra; Silva Carvalho, JL (Autor) Outra; Almeida, H (Autor) FMUP

Revista

Título: Molecular Human Reproduction

Vol. 15 Nº 7

Páginas: 411-419

ISSN: 1360-9947

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 85 Citações

ISI Web of Science

Scopus - 96 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-HZM

DOI: 10.1093/molehr/gap034

Abstract (EN): Success in assisted reproductive techniques (ART) is influenced by gamete and embryo quality but the assessment of these parameters has been thwarted by the lack of reliable biomarkers. Follicular fluid and cumulus oophorus cells may provide biomarkers due to their close relationship to the oocyte. These cells produce antioxidants and thus protect the oocyte from oxidative damage exerted by reactive oxygen species (ROS). ROS and antioxidants are known to intervene in reproductive physiology and pathology, but their roles are unclear. It is hypothesized that superoxide dismutase (SOD), a first line antioxidant enzyme, is associated with oocyte quality. Cells obtained in the course of ART for the treatment of infertility due to male factor or female pathology were processed for SOD intracellular isoforms (CuZnSOD and MnSOD) immunodetection, total SOD activity and isoforms content. Cells presented strong positive staining for CuZnSOD and MnSOD. SOD activity decreased with increasing female age but was increased in endometriosis and in ovulatory dysfunction. When male factor was the cause for infertility, successful ART was associated with higher SOD activity. Variations in SOD emphasize the relevance of oxidative stress in the oocyte maturation process. These variations also suggest that SOD is a potential biomarker for ART success.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9