Optimization and validation of organochlorine compounds in adipose tissue by SPE-gas chromatography

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Virginia C Fernandes (Autor) Outra; Diogo Pestana (Autor) Outra; Rosario Monteiro (Autor) FMUP; Gil Faria (Autor) Outra; Manuela Meireles (Autor) FMUP; Luisa Correia Sa (Autor) Outra; Diana Teixeira (Autor) Outra; Ana Faria (Autor) FCUP; Conceicao Calhau (Autor) FMUP; Valentina F Domingues (Autor) Outra; Cristina Delerue Matos (Autor) Outra

Revista

Título: Biomedical Chromatography

Vol. 26

Páginas: 1494-1501

ISSN: 0269-3879

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 15 Citações

ISI Web of Science

Scopus - 17 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química > Análise instrumental

Outras Informações

ID Authenticus: P-002-365

DOI: 10.1002/bmc.2723

Abstract (EN): Scientific evidence has shown an association between organochlorine compounds (OCC) exposure and human health hazards. Concerning this, OCC detection in human adipose samples has to be considered a public health priority. This study evaluated the efficacy of various solid-phase extraction (SPE) and cleanup methods for OCC determination in human adipose tissue. Octadecylsilyl endcapped (C18-E), benzenesulfonic acid modified silica cation exchanger (SA), poly(styrene-divinylbenzene (EN) and EN/RP18 SPE sorbents were evaluated. The relative sample cleanup provided by these SPE columns was evaluated using gas chromatography with electron capture detection (GCECD). The C18-E columns with strong homogenization were found to provide the most effective cleanup, removing the greatest amount of interfering substance, and simultaneously ensuring good analyte recoveries higher than 70%. Recoveries?>?70% with standard deviations (SD)?<?15% were obtained for all compounds under the selected conditions. Method detection limits were in the 0.0030.009?mg/kg range. The positive samples were confirmed by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The highest percentage found of the OCC in real samples corresponded to HCB, o,p'-DDT and methoxychlor, which were detected in 80 and 95% of samples analyzed respectively. Copyright (c) 2012 John Wiley & Sons, Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

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Nº de páginas: 8