Solving the Catalytic Mechanism of Tryptophan Synthase: an Emergent Drug Target in the Treatment of Tuberculosis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Teixeira, CSS (Autor) Outra; Ramos, MJ (Autor) FCUP; Sergio Filipe Sousa (Autor) FCUP; Nuno M F S A Cerqueira (Autor) FCUP

Revista

Título: ChemCatChem

Vol. 12 Nº 4

Páginas: 227-237

ISSN: 1867-3880

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 9 Citações

Outras Informações

ID Authenticus: P-00R-935

DOI: 10.1002/cctc.201901505

Abstract (EN): Tryptophan Synthase (TSase) is an emergent therapeutic target in the treatment of tuberculosis. Interest in TSase as a drug target arose from the fact that this enzyme is not present in humans, while in bacteria, like M. tuberculosis, it catalyses the last two steps in the tryptophan biosynthetic pathway. Several inhibitors of TSase have recently been developed, with promising results. However, the exact catalytic mechanism of this enzyme has remained unexplained at the atomic level. The fact that TSase is a multifunctional enzyme, with two dimers, each one with two independent active sites, interconnected by a 25 angstrom tunnel, has made it a challenging enzyme, from the catalytic point of view. QM/MM calculations were used to analyze and explain the two steps catalyzed by this enzyme. The results provide an atomic-level clarification of the full catalytic mechanism of this enzyme, offering also important clues for the development of new inhibitors against tuberculosis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11