The effect of high glucose on SERT, the human plasmalemmal serotonin transporter

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Gonçalves P (Autor) FMUP; Araújo JR (Autor) FMUP; Martel, F (Autor) FMUP

Revista

Título: Nutritional Neuroscience

Vol. 11

Páginas: 244-250

ISSN: 1028-415X

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-003-TPS

DOI: 10.1179/147683008x344156

Abstract (EN): The aim of this work was to investigate the effect of short- and long-term high-glucose exposure on the plasmalemmal serotonin transporter (SERT)-mediated uptake of [(3)H]-serotonin (5-HT) by Caco-2 cells. Short-term exposure of Caco-2 cells to high apical glucose levels (30 mM for 2 h or 40 mM for 1 h) decreased the uptake of [(3)H]-5-HT by 20-30%. On the other hand, long-term (21-24 weeks) exposure of the cells to high (25 mM) glucose caused a 30% increase in the uptake of [(3)H]-5-HT. Under these conditions, the affinity of the transporter for 5-HT and noradrenaline was not significantly changed, and the inhibitory potencies of fluoxetine and desipramine were also unchanged. In conclusion, high-glucose levels modulate SERT activity. A short- term exposure of the cells to a high concentration of glucose decreases the activity of the transporter, whereas a longer exposure of the cells to a high concentration of glucose increases the activity of SERT, without interfering with its affinity.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7