Oxidative stress decreases uptake of neutral amino acids in a human placental cell line (BeWo cells)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Araújo JR (Autor) FMUP; Correia Branco, A (Autor) Outra; Pereira, AC (Autor) Outra; Maria João Pinho (Autor) FMUP; Elisa Keating (Autor) FMUP; Martel, F (Autor) FMUP

Revista

Título: Reproductive Toxicology

Vol. 40

Páginas: 76-81

ISSN: 0890-6238

Editora: Elsevier

Indexação

ISI Web of Knowledge - 24 Citações

Scopus - 27 Citações

Outras Informações

ID Authenticus: P-006-7ZD

DOI: 10.1016/j.reprotox.2013.06.073

Abstract (EN): Increased oxidative stress (OS) is implicated in the pathophysiology of several pregnancy disorders. We aimed to investigate the effect of tert-butylhydroperoxide (TBHP)-induced OS upon the placental transport of the neutral amino acids L-methionine (L-Met) and L-alanine (L-Ala), by using a human trophoblast cell model (BeWo cells). TBHP reduced both total and Ne-independent C-14-L-Met intracellular steady-state accumulation over time (A(max)), by reducing non-system L-mediated uptake - most probably system y(+) while having no effect on system L. Moreover, TBHP reduced total C-14-L-Ala A(max) through an inhibition of system A. The effect of TBHP upon total, but not system A-mediated, C-14-L-Ala uptake was dependent upon phosphoinositide 3-kinase (PI3K) and protein kinase C (PKC) activation, and was completely prevented by the polyphenol quercetin. In conclusion, a reduction in placental uptake of neutral amino acids may contribute to the deleterious effects of pregnancy disorders associated with OS.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6