Two-dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibility

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

vitorino, r (Autor) Outra; guedes, sdm (Autor) Outra; ferreira, r (Autor) Outra; lobo, mjc (Autor) Outra; duarte, j (Autor) FADEUP; ferrer-correia, aj (Autor) Outra; tomer, kb (Autor) Outra; domingues, pm (Autor) Outra; amado, fml (Autor) Outra

Revista

Título: European Journal of Oral Sciences

Vol. 114 Nº 2

Páginas: 147-153

ISSN: 0909-8836

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 79 Citações

Scopus - 84 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-004-MDS

DOI: 10.1111/j.1600-0722.2006.00328.x

Abstract (EN): In the present study, a proteomic approach was applied to evaluate the influence of salivary protein composition on in vitro dental pellicle formation and its possible correlation with dental caries. Whole saliva, collected from caries-free and caries-susceptible subjects, was analyzed by two-dimensional electrophoresis, and protein spots were identified by mass spectrometry. Data analysis of salivary protein composition showed a statistically significant correlation between the quantity of acidic proline-rich proteins (PRPs), lipocalin, cystatin SN and cystatin S, and samples from the caries-free group of subjects [decayed, missing or filled teeth (DMFT) = 0]. Samples from subjects with a high DMFT index appear to be correlated with high levels of amylase, immunoglobulin A, and lactoferrin. In vitro pellicle-composition experiments showed the same correlations found for whole saliva. As cystatins are known physiological inhibitors of cathepsins, the higher quantities of lipocalin, and cystatins S and SN found in the samples from the caries-free subjects suggest that inhibition of proteolytic events on other salivary proteins may indirectly provide tooth protection. The correlation between higher levels of the phosphorylated acidic PRPs 1/2 with samples from the caries-free group also suggests a protective role for these proteins.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7