Immunophenotype and TCR-V beta repertoire of peripheral blood T-cells in acute infectious mononucleosis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Lima, M (Autor) Outra; Teixeira, MD (Autor) Outra; Queiros, ML (Autor) Outra; Santos, AH (Autor) Outra; Goncalves, C (Autor) Outra; Correia, J (Autor) Outra; Farinha, F (Autor) Outra; Mendonca, F (Autor) Outra; Soares, JMN (Autor) Outra; Almeida, J (Autor) Outra; Orfao, A (Autor) Outra; Justica, B (Autor) Outra

Revista

Título: Blood Cells, Molecules, and Diseases

Vol. 30

Páginas: 1-12

ISSN: 1079-9796

Editora: Elsevier

Indexação

ISI Web of Knowledge - 24 Citações

Scopus - 25 Citações

Outras Informações

ID Authenticus: P-000-J8S

DOI: 10.1016/s1079-9796(03)00014-7

Abstract (EN): Although a number of studies on the phenotypic changes that occur after T-cell activation have already been published, the specific immunophenotypic features of T-lymphocytes and the frequency at which TCR-variable region (TCR-V) restricted T-cell expansions occur "in vivo" during acute viral infection still remains to be established. We report on the immunophenotype and TCR-V repertoire of peripheral blood T-cells from 28 patients with acute infectious mononucleosis. Immunophenotypic studies were performed by flow cytometry using direct immunofluorescence techniques and stain-and-then-lyse sample preparation protocols with three- and four-colour combinations of monoclonal antibodies directed against a large panel of T- and NK-cell associated markets, activation- and adhesion-related molecules and TCR-Vbeta, -Vgamma and -Vdelta families. Nearly all patients (27/28) showed a massive expansion of CD8(+)/TCRalphabeta(+) T cells, the majority (>90%) of which displayed an immunophenotype compatible with T-cell activation: CD2(+high) CD7(+low), CD11a(+high), CD38(+high), HLA-DR+high, CD28(+/-low), CD45RO(+high), CD45RA(-/+low), CD11b(-/+low), CD11c(+/-low), CD16(-), CD56(-), CD57(-),CD62L(-),CD94(-),CD158a(-),CD161(-),NKB1(-). Additionally, the levels of both CD3 and CD5 were slightly decreased compared to those found in normal individuals. Late-activation antigens, such as CD57, were found in small proportions of CD8(+)/rCRalphabeta(+) T-cells. Increased numbers of CD4(+)/TCRalphabeta(+) T-cells, TCRgammadelta(+) T-cells and NK-cells were also noticed in 17, 16 and 13 of the 28 cases studied, respectively. Evidence for activation of CD4(+)/rCRalphabeta(+) and TCRgammadelta(+) T-cells relied on changes similar to those described for CD8(+)/TCRalphabeta(+) although less pronounced, except for higher levels of both CD5 and CD28 in the absence of reactivity for CD11c on CD4+/TCRalphabeta(+) T-cells and higher levels of CD161 and CD94 on TCRgammadelta(+) T-cells. Small expansions of one or more TCR-Vbeta families accounting for 12 +/- 7% of either the CD8(+)/TCRalphabeta(+) or the CWITCRap' T-cell compartment were found in 12 of 14 patients studied, whereas the distribution of the TCR-Vgamma and -Vdelta repertoires tested in 2 of the individuals with expanded TCRgammadelta(+) T-cells was similar to that observed in control individuals. The results presented here provide evidence for an extensive T-cell activation during acute viral infection and establish the immunophenotype patterns associated with this condition.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12