Profiling DNA Methylation Based on Next-Generation Sequencing Approaches: New Insights and Clinical Applications

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Barros Silva, D (Autor) Outra; Joana Marques, CJ (Autor) FMUP; henrique, rmf (Autor) ICBAS; Carmen Jeronimo (Autor) ICBAS

Revista

Título: Genes

Vol. 9

Página Final: 429

ISSN: 2073-4425

Editora: MDPI

Indexação

ISI Web of Knowledge - 85 Citações

Scopus - 109 Citações

Outras Informações

ID Authenticus: P-00P-K06

DOI: 10.3390/genes9090429

Abstract (EN): DNA methylation is an epigenetic modification that plays a pivotal role in regulating gene expression and, consequently, influences a wide variety of biological processes and diseases. The advances in next-generation sequencing technologies allow for genome-wide profiling of methyl marks both at a single-nucleotide and at a single-cell resolution. These profiling approaches vary in many aspects, such as DNA input, resolution, coverage, and bioinformatics analysis. Thus, the selection of the most feasible method according with the project's purpose requires in-depth knowledge of those techniques. Currently, high-throughput sequencing techniques are intensively used in epigenomics profiling, which ultimately aims to find novel biomarkers for detection, diagnosis prognosis, and prediction of response to therapy, as well as to discover new targets for personalized treatments. Here, we present, in brief, a portrayal of next-generation sequencing methodologies' evolution for profiling DNA methylation, highlighting its potential for translational medicine and presenting significant findings in several diseases.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12