Influence of chronological aging on the survival and nucleotide content of Saccharomyces cerevisiae cells grown in different conditions: occurrence of a high concentration of UDP-N-acetylglucosamine in stationary cells grown in 2% glucose

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Osorio, H (Autor) Outra; Silles, E (Autor) Outra; Maia, R (Autor) Outra; peleteiro, b (Autor) Outra; Pedro Moradas Ferreira (Autor) ICBAS; Sillero, MAG (Autor) Outra; Sillero, A (Autor) Outra

Revista

Título: FEMS Yeast Research

Vol. 5

Páginas: 387-398

ISSN: 1567-1356

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 9 Citações

Outras Informações

ID Authenticus: P-000-4V9

DOI: 10.1016/j.femsyr.2004.10.001

Abstract (EN): Saccharomyces cerevisiae cells (strain W303) grown in a minimal medium (containing 2% or 0.1% glucose) until exponential or stationary phase, were subjected to chronological aging in water, and yeast viability and nucleotide content were analyzed along several days of nutrient starvation. Cells collected in exponential phase (whether grown in the presence of 0.1% or 2% glucose) were viable up to five days and thereafter the viability decreased linearly with a half-survival rate of around eight days. ATP and other nucleoside triphosphates decreased similarly in both cases. Cells collected in stationary phase, and transferred to water, behaved differently whether grown in 0.1% or in NO glucose, with a half-survival life of around nine and 28 days respectively. A double mutant in glycogen synthase (gsy1Delta gsy2Delta) and its isogenic wild-type strain, grown to stationary phase in 2% glucose, presented a similar half-survival life of around eight days. The W303 cells grown to stationary phase in the presence of NO glucose showed a 7-fold increase of UDP-N-acetylglucosamine (UDP-GlcNAc) as compared with the level present in the cells grown in any of the other three metabolic situations. The nature of UDP-GlcNAc was established by MALDI-TOF ionization analysis. It is also worth noting that the rate of decay of NAD+ was lower than that of ATP in any of the situations here considered.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12