A Systematic AQbD Approach for Optimization of the Most Influential Experimental Parameters on Analysis of Fish Spoilage-Related Volatile Amines

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Freitas, J (Autor) Outra; Silva, P (Autor) Outra; vaz-pires, p (Autor) ICBAS; Camara, JS (Autor) Outra

Revista

Título: Foods

Vol. 9

Página Final: 1321

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00S-R9M

DOI: 10.3390/foods9091321

Abstract (EN): The volatile amines trimethylamine (TMA) and dimethylamine (DMA) could be used as important spoilage indices for seafood products, assisting in the determination of the rejection period. In the present study, a systematic analytical duality-by-design (AQbD) approach was used as a powerful strategy to optimize the most important experimental parameters of headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) conditions for the quantification of TMA and DMA inSparus aurata. This optimization enabled the selection of the best points in the method operable design region for HS-SPME extraction (30 min; 35 degrees C; NaOH 15 M and NaCl 35%,w/v) and GC-MS analysis (80 degrees C; gradient 50 degrees C/min; flow rate 1 mL/min and splitless mode). The rejection day, estimated through the TMA concentration (>12 mg/100 g, at days 9-10), was compared with sensory (quality index method: day 7-8), physical (Torrymeter: day 8-9), and microbial (day 9-10) analysis, corroborating the suitability of the proposed approach for estimating the period for which they will retain an acceptable level of eating quality from a safety and sensory perspective.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17