Inhibitory Effect of 5-Aminoimidazole-4-Carbohydrazonamides Derivatives Against Candida spp. Biofilm on Nanohydroxyapatite Substrate

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Gabriel, C (Autor) Outra; Liliana Grenho (Autor) FMDUP; Cerqueira, F (Autor) Outra; Medeiros, R (Autor) Outra; Dias, AM (Autor) Outra; Ribeiro, AI (Autor) Outra; Proenca, MF (Autor) Outra; Fernandes MH (Autor) FMDUP; Sousa, JC (Autor) Outra; Fernando Mendes Monteiro (Autor) FEUP; Ferraz, MP (Autor) Outra

Revista

Título: Mycopathologia

Vol. 184

Páginas: 775-786

ISSN: 0301-486X

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 7 Citações

Outras Informações

ID Authenticus: P-00R-90J

DOI: 10.1007/s11046-019-00400-4

Abstract (EN): Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N '-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating. [GRAPHICS] .

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12