Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Pinho, AC (Autor) Outra; Branquinho, MV (Autor) Outra; Alvites, RD (Autor) Outra; Fonseca, AC (Autor) Outra; Caseiro, AR (Autor) Outra; Pedrosa, SS (Autor) Outra; luis, al (Autor) ICBAS; Pires, I (Autor) Outra; Prada, J (Autor) Outra; Muratori, L (Autor) Outra; Ronchi, G (Autor) Outra; Geuna, S (Autor) Outra; Santos, JD (Autor) FEUP; Mauricio, AC (Autor) ICBAS; Serra, AC (Autor) Outra; Jordao Coelho, JFJ (Autor) Outra

Revista

Título: Biomaterials Science

Vol. 8

Páginas: 798-811

ISSN: 2047-4830

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 5 Citações

Outras Informações

ID Authenticus: P-00R-KHF

DOI: 10.1039/c9bm00901a

Abstract (EN): In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly(epsilon-caprolactone) (PCL). Swelling capacity of the tube-guides ranged from 40-60% with no visible constriction of their inner diameter. In vitro hydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The in vivo performance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of Tibialis anterior (TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14