Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Freire, AG (Autor) Outra; Waghray, A (Autor) Outra; Soares da Silva, F (Autor) Outra; Resende, TP (Autor) Outra; Lee, DF (Autor) Outra; Pereira, CF (Autor) Outra; Nascimento, DS (Autor) ICBAS; Lemischka, IR (Autor) Outra; Perpetua Pinto do O (Autor) ICBAS

Revista

Título: Stem Cell Reports

Vol. 9

Páginas: 136-148

ISSN: 2213-6711

Editora: Elsevier

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 3 Citações

Outras Informações

ID Authenticus: P-00R-AQ2

DOI: 10.1016/j.stemcr.2017.05.025

Abstract (EN): Notch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13