"Out of the Can": A Draft Genome Assembly, Liver Transcriptome, and Nutrigenomics of the European Sardine, Sardina pilchardus

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Machado, AM (Autor) Outra; Torresen, OK (Autor) Outra; Kabeya, N (Autor) Outra; Couto, A (Autor) Outra; Petersen, B (Autor) Outra; Felicio, M (Autor) Outra; Campos, PE (Autor) Outra; Fonseca, E (Autor) Outra; Bandarra, N (Autor) Outra; Lopes Marques, M (Autor) Outra; Ferraz, R (Autor) Outra; Ruivo, R (Autor) Outra; Fonseca, MM (Autor) Outra; Jentoft, S (Autor) Outra; Monroig, O (Autor) Outra; da Fonseca, RR (Autor) Outra; Filipe F C Castro (Autor) FCUP

Revista

Título: Genes

Vol. 9

Página Final: 485

ISSN: 2073-4425

Editora: MDPI

Indexação

ISI Web of Knowledge - 23 Citações

Scopus - 27 Citações

Outras Informações

ID Authenticus: P-00P-TC3

DOI: 10.3390/genes9100485

Abstract (EN): Clupeiformes, such as sardines and herrings, represent an important share of worldwide fisheries. Among those, the European sardine (Sardina pilchardus, Walbaum 1792) exhibits significant commercial relevance. While the last decade showed a steady and sharp decline in capture levels, recent advances in culture husbandry represent promising research avenues. Yet, the complete absence of genomic resources from sardine imposes a severe bottleneck to understand its physiological and ecological requirements. We generated 69 Gbp of paired-end reads using Illumina HiSeq X Ten and assembled a draft genome assembly with an N50 scaffold length of 25,579 bp and BUSCO completeness of 82.1% (Actinopterygii). The estimated size of the genome ranges between 655 and 850 Mb. Additionally, we generated a relatively high-level liver transcriptome. To deliver a proof of principle of the value of this dataset, we established the presence and function of enzymes (Elov12, Elov15, and Fads2) that have pivotal roles in the biosynthesis of long chain polyunsaturated fatty acids, essential nutrients particularly abundant in oily fish such as sardines. Our study provides the first omics dataset from a valuable economic marine teleost species, the European sardine, representing an essential resource for their effective conservation, management, and sustainable exploitation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13