Using Cell Cultures for the Investigation of Treatments for Attention Deficit Hyperactivity Disorder: A Systematic Review

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2019

Autores

Chierrito, D (Autor) Outra; Villas Boas, CB (Autor) Outra; Tonin, FS (Autor) Outra; Fernandez Llimos, F (Autor) Outra; Sanches, ACC (Autor) Outra; de Mello, JCP (Autor) Outra

Revista

Título: Current Neuropharmacology

Vol. 17

Páginas: 916-925

ISSN: 1570-159X

Editora: Bentham Science

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-00R-3Q9

DOI: 10.2174/1570159x17666190409143155

Abstract (EN): Background: Advances in basic and molecular biology have promoted the use of cell cultures in a wide range of areas, including the evaluation of drug efficacy, safety and toxicity. Objective This article aims to provide a general overview of the methodological parameters of cell cultures used to investigate therapeutic options for Attention Deficit Hyperactivity Disorder. Method: A systematic search was performed in the electronic databases PubMed, Scopus, and DOAJ. In vitro experimental studies using cell cultures were included. Results: A total of 328 studies were initially identified, with 16 included for qualitative synthesis. Seven studies used neuronal cells (SH-SY5Y neuroblastoma and PC12 cell line) and nine used non-neuronal cells. All the studies described the culture conditions, but most studies were inconsistent with regard to reporting results and raw data. Only one-third of the studies performed cell viability assays, while a further 30% conducted gene expression analysis. Other additional tests included electrophysiological evaluation and transporter activity. More than 50% of the studies evaluated the effects of drugs such as methylphenidate and atomoxetine, while plant extracts were assessed in four studies and polyunsaturated fatty acids in one. Conclusion: We suggested a flowchart to guide the planning and execution of studies, and a checklist to be completed by authors to allow the standardized reporting of results. This may guide the elaboration of laboratory protocols and further in vitro studies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10