Endogenous adenosine modulation of Na-22 uptake by rat brain synaptosomes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Ribeiro, JA (Autor) Outra; Lobo, MGB (Autor) ICBAS; Sebastiao, AM (Autor) Outra

Revista

Título: Neurochemical Research

Vol. 28

Páginas: 1591-1595

ISSN: 0364-3190

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 2 Citações

Scopus - 2 Citações

Outras Informações

ID Authenticus: P-000-F22

DOI: 10.1023/a:1025638929136

Abstract (EN): To evaluate if endogenous extracellular adenosine influences sodium channel activity in nerve terminals, we investigated how manipulations of extracellular adenosine levels influence Na-22 uptake by rat brain synaptosomes stimulated with veratridine (VT). To decrease extracellular adenosine levels, adenosine deaminase (ADA) that converts adenosine into an inactive metabolite was used. To increase extracellular adenosine levels, we used the adenosine deaminase inhibitor erythro-9(2-hydroxy-3-nonyl) adenine ( EHNA), as well as the inhibitor of adenosine transport, nitrobenzylthioinosine ( NBTI). ADA ( 0.1-5 U/ml) caused an excitatory effect on 22Na uptake stimulated by veratridine, which was abolished in the presence of the adenosine deaminase inhibitor erythro-9(2-hydroxy-3-nonyl) adenine ( EHNA, 25 muM). Both the adenosine uptake inhibitor nitrobenzylthioinosine (NBTI, 1 - 10 muM) and the adenosine deaminase inhibitor EHNA (10 - 25 muM) inhibited Na-22 uptake by rat brain synaptosomes. It is suggested that adenosine is tonically inhibiting sodium uptake by rat brain synaptosomes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5