Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Oliveira, BM (Autor) Outra; Pinto, A (Autor) Outra; Alexandra Correia (Autor) ICBAS; ferreira, pg (Autor) ICBAS; Manuel Vilanova (Autor) ICBAS; Teixeira, L (Autor) ICBAS

Revista

Título: Scientific Reports

Vol. 10

ISSN: 2045-2322

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 10 Citações

Projetos Associados

ADIPONEO - Estudo da resposta imunológica no tecido adiposo de hospedeiros infectados com Neospora Caninum

Outras Informações

ID Authenticus: P-00R-P89

DOI: 10.1038/s41598-020-58678-0

Abstract (EN): Immune cells resident in adipose tissue have important functions in local and systemic metabolic homeostasis. Nevertheless, these immune cell populations remain poorly characterized in bovines. Recently, we described diverse lymphocyte subpopulations in adipose tissue of Holstein-Friesian cows. Here, we aimed at characterising myeloid cell populations present in bovine adipose tissue using multicolour flow cytometry, cell sorting and histochemistry/immunohistochemistry. Macrophages, CD14(+)CD11b(+)MHC-II(+)CD45(+) cells, were identified in mesenteric and subcutaneous adipose tissue, though at higher proportions in the latter. Mast cells, identified as SSC-A(high)CD11b(-/+)CD14(-)MHC-II(-)CH138A(-)CD45(+) cells, were also observed in adipose tissue and found at higher proportions than macrophages in mesenteric adipose tissue. Neutrophils, presenting a CH138A(+)CD11b(+) phenotype, were also detected in mesenteric and subcutaneous adipose tissue, however, at much lower frequencies than in the blood. Our gating strategy allowed identification of eosinophils in blood but not in adipose tissue although being detected by morphological analysis at low frequencies in some animals. A population not expressing CD45 and with the CH138A(+) CD11b(-)MHC-II- phenotype, was found abundant and present at higher proportions in mesenteric than subcutaneous adipose tissue. The work reported here may be useful for further studies addressing the function of the described cells.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15