Genetic variation in the conjugative plasmidome of a hospital effluent multidrug resistant Escherichia coli strain

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Catarina Ferreira (Autor) Outra; Diana Bogas (Autor) Outra; Santosh K. Bikarolla (Autor) Outra; Ana Rita Varela (Autor) Outra; Karolin Frykholm (Autor) Outra; Raquel Linheiro (Autor) Outra; Olga C. Nunes (Autor) FEUP; Fredrik Westerlund (Autor) Outra; Célia M. Manaia (Autor) Outra

Revista

Título: Chemosphere

Vol. 220

Páginas: 748-759

ISSN: 0045-6535

Editora: Elsevier

Indexação

ISI Web of Knowledge - 1 Citação

ISI Web of Science

Scopus - 6 Citações

Projetos Associados

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00Q-808

DOI: 10.1016/j.chemosphere.2018.12.130

Abstract (EN): Bacteria harboring conjugative plasmids have the potential for spreading antibiotic resistance through horizontal gene transfer. It is described that the selection and dissemination of antibiotic resistance is enhanced by stressors, like metals or antibiotics, which can occur as environmental contaminants. This study aimed at unveiling the composition of the conjugative plasmidome of a hospital effluent multidrug resistant Escherichia coli strain (H1FC54) under different mating conditions. To meet this objective, plasmid pulsed field gel electrophoresis, optical mapping analyses and DNA sequencing were used in combination with phenotype analysis. Strain H1FC54 was observed to harbor five plasmids, three of which were conjugative and two of these, pH1FC54_330 and pH1FC54_140, contained metal and antibiotic resistance genes. Transconjugants obtained in the absence or presence of tellurite (0.5 mu M or 5 mu M), arsenite (0.5 mu M, 5 mu M or 15 mu M) or ceftazidime (10 mg/L) and selected in the presence of sodium azide (100 mg/L) and tetracycline (16 mg/L) presented distinct phenotypes, associated with the acquisition of different plasmid combinations, including two co-integrate plasmids, of 310 kbp and 517 kbp. The variable composition of the conjugative plasmidome, the formation of co-integrates during conjugation, as well as the transfer of non-transferable plasmids via co-integration, and the possible association between antibiotic, arsenite and tellurite tolerance was demonstrated. These evidences bring interesting insights into the comprehension of the molecular and physiological mechanisms that underlie antibiotic resistance propagation in the environment. (C) 2018 Published by Elsevier Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12