Online HPLC-DPPH screening method for evaluation of radical scavenging phenols extracted from Moringa oleifera leaves

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Braham, F (Autor) Outra; Carvalho, DO (Autor) FCUP; Marisa M R Almeida (Autor) FCUP; Zaidi, F (Autor) Outra; Magalhaes, JMCS (Autor) Outra; Luis F Guido (Autor) FCUP; Goncalves, MP (Autor) Outra

Revista

Título: South African Journal of Botany

Vol. 129

Páginas: 146-154

ISSN: 0254-6299

Editora: Elsevier

Indexação

ISI Web of Knowledge - 23 Citações

Scopus - 22 Citações

Outras Informações

ID Authenticus: P-00Q-KDM

DOI: 10.1016/j.sajb.2019.04.001

Abstract (EN): In this work the phenolic profile of Moringa oleifera leaves from trees located in Oued Souf and Tamanrasset, desert region south Algeria, was characterized by a real-time HPLC coupled to the DPPH assay, together with high resolution mass spectrometry (HPLC-DAD-ESI-MSn) studies. The contribution of single component to the overall radical scavenging capacity was evaluated for water, hydroalcoholic and hydroacetonic extracts of M. oleifera leaves. Hydroacetonic extracts (70% acetone) exhibit the highest amount of phenolic compounds (57 +/- 3 mg GAE/g), as well as the highest flavonoid content (9.12 +/- 0.01 mg QE/g). Twelve compounds, mainly phenolics, were identified by high-resolution orbitrapmass spectrometry. Among these, chlorogenic acids (3-caffeoylquinic acid and 4-caffeoylquinic acid) and quercetin glycosides (quercetin-3-O-glucoside, quercetin-3-O-galactoside and quercetin 3-O-(6 ''-malonyl) glucoside) are the most potent quenchers of DPPH radical both for hydroethanolic and hydroacetonic extracts. Aqueous extracts exhibit a quite different behaviour, with citric acid and quinic acid, arising from the oxidation of the chlorogenic acids, acting as the unique antioxidants.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9