Comparison of five bacterial strains producing siderophores with ability to chelate iron under alkaline conditions

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Carlos M. H. Ferreira (Autor) Outra; Ângela Vilas Boas (Autor) Outra; Cátia A. Sousa (Autor) Outra; Helena Soares (Autor) FEUP; Helena M. V. M. Soares (Autor) Outra

Revista

Título: AMB Express

Vol. 9

Páginas: 1-12

ISSN: 2191-0855

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 25 Citações

Outras Informações

ID Authenticus: P-00Q-MXD

DOI: 10.1186/s13568-019-0796-3

Abstract (EN): Iron deficiency is one of the main causes of chlorosis in plants, which leads to losses in field crops quality and yield. The use of synthetic chelates to prevent or correct iron-deficiency is not satisfactory mainly due to their poor biodegradability. The present work aimed to search suitable microorganisms to produce alternative, environment-friendly iron-chelating agents (siderophores). For this purpose, the performance of five bacteria (Azotobacter vinelandii, Bacillus megaterium, Bacillus subtilis, Pantoea allii and Rhizobium radiobacter) was evaluated, regarding siderophore production kinetics, level of siderophore production (determined by chrome azurol S, CAS method), typeof siderophore produced (using Arnow and Csaky's tests) and iron-chelating capacity at pH 9.0. All bacteria were in stationary phase at 24h, except A. vinelandii (at 72h) and produced the maximum siderophore amount (80-140 mu molL(-1)) between 24 and 48h, with the exception of A. vinelandii (at 72h). The analysis of culture filtrates revealed the presence of catechol-type siderophores for B. subtilis and R. radiobacter and hydroxamate-type siderophores for B. megaterium and P. allii. In the case of A. vinelandii, both siderophore-types (catechol and hydroxamates) were detected. The highest iron-chelating capacity, at pH 9.0, was obtained by B. megaterium followed by B. subtilis and A. vinelandii. Therefore, these three bacteria strains are the most promising bacteria for siderophore production and chlorosis correction under alkaline conditions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12