NK and NKT cells in the diagnosis of diffuse lung diseases presenting with a lymphocytic alveolitis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Sokhatska, O (Autor) FMUP; Padrao, E (Autor) Outra; Sousa Pinto, B (Autor) FMUP; Beltrao, M (Autor) Outra; Mota, PC (Autor) Outra; Melo, N (Autor) Outra; Delgado, L (Autor) FMUP; Morais, A (Autor) FMUP

Revista

Título: BMC Pulmonary Medicine

Vol. 19

ISSN: 1471-2466

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00Q-79S

DOI: 10.1186/s12890-019-0802-1

Abstract (EN): Background: Diffuse lung diseases (DLD) are characterized by different immunophenotypes in the bronchoalveolar lavage fluid (BALF). We aimed to evaluate the diagnostic value of BALF NK and NKT cell counts of patients with DLD and lymphocytic alveolitis. Methods: We assessed 202 patients with DLD, who underwent BALF immunophenotyping. Samples were routinely processed by flow cytometry and lymphocyte subsets were compared between patients with sarcoidosis (n=106), hypersensitivity pneumonitis (HP; n=53), and other DLDs (n=43). We compared absolute counts and percentages of NK and NKT cells between patients with HP versus the remaining DLD patients. To assess the accuracy of BALF lymphocyte subsets in the diagnosis of HP, we calculated the respective areas under the receiver operating characteristic curves (AUC-ROC). Results: Patients with HP had significantly higher numbers of BALF NK cells, and its percentage was significantly associated with a higher odds of HP, even after adjustment for the NKT and CD8(+) cells. For the absolute number of BALF NK cells, we found an AUC-ROC of 0.76 (95%CI=0.68-0.84) when comparing patients with HP versus the remaining DLD. The cut-offs of 2000 NK cells/mL and of 2.4% NK cells in the BALF had a specificity and a negative predictive value over 80% for the diagnosis of HP. BALF NK cells absolute counts were significantly higher in HP patients with a restrictive pattern. No such differences were observed for NKT cells. Conclusions: BALF NK immunophenotyping may be a helpful adjunct to the diagnostic work-up of DLD, particularly in the differential diagnosis of HP.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10