A mathematical model of the proton balance in the outer mantle epithelium of Anodonta cygnea L.

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Oliveira, PF (Autor) Outra; da Costa, AR (Autor) Outra; Ferreira, HG (Autor) Outra

Revista

Título: Journal of Membrane Biology

Vol. 223

Páginas: 59-72

ISSN: 0022-2631

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 2 Citações

Scopus - 2 Citações

Outras Informações

ID Authenticus: P-003-ZS7

DOI: 10.1007/s00232-008-9114-8

Abstract (EN): In the freshwater mollusc Anodonta cygnea and other unionids, the mantle plays an important role in the regulation of the movements of ions between the shell and the extrapaleal fluid. In this report, a mathematical model that attempts to describe the cell metabolic mechanisms underlying the operation of the outer mantle epithelium as a source of protons is presented. We encoded the information gathered by studying the epithelium in vitro, which includes the electrophysiology of the preparation, measurements of basic rates of transport of protons and base, the effect of metabolic and transport inhibitors on its electrical behavior and the dynamic measurements of pH(i) . The model was conceived so that the short-circuit current (Isc) and fluxes of Na(+), K(+) and Cl(-); intracellular volume; electrical potential; and ionic concentrations can be computed as a function of time. Furthermore, the analytical descriptions of all ionic fluxes involved are such that the effect of transport inhibitors can be simulated. In all the simulations performed, it was possible to reproduce the experimental results obtained with specific inhibitors of transport systems on the Isc and on pH (i) . In some cases, it was necessary to make alterations to one or more parameters of the reference condition. For each simulation carried out, the analysis of the results was consistent. The model is an analytical tool that can be used to show the internal coherence of the qualitative model previously proposed and to plan further experiments.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14