Whole-Genome Identification, Phylogeny, and Evolution of the Cytochrome P450 Family 2 (CYP2) Subfamilies in Birds

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Almeida, D (Autor) Outra; Maldonado, E (Autor) Outra; Khan, I (Autor) Outra; Silva, L (Autor) Outra; Gilbert, MTP (Autor) Outra; Zhang, GJ (Autor) Outra; Jarvis, ED (Autor) Outra; O'Brien, SJ (Autor) Outra; Johnson, WE (Autor) Outra; Agostinho Antunes (Autor) FCUP

Revista

Título: Genome Biology and Evolution

Vol. 8

Páginas: 1115-1131

ISSN: 1759-6653

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 21 Citações

Scopus - 22 Citações

Outras Informações

ID Authenticus: P-00K-GCD

DOI: 10.1093/gbe/evw041

Abstract (EN): The cytochrome P450 (CYP) superfamily defends organisms from endogenous and noxious environmental compounds, and thus is crucial for survival. However, beyond mammals the molecular evolution of CYP2 subfamilies is poorly understood. Here, we characterized the CYP2 family across 48 avian whole genomes representing all major extant bird clades. Overall, 12 CYP2 subfamilies were identified, including the first description of the CYP2F, CYP2G, and several CYP2AF genes in avian genomes. Some of the CYP2 genes previously described as being lineage-specific, such as CYP2K and CYP2W, are ubiquitous to all avian groups. Furthermore, we identified a large number of CYP21 copies, which have been associated previously with water reabsorption. We detected positive selection in the avian CYP2C, CYP2D, CYP2H, CYP2J, CYP2K, and CYP2AC subfamilies. Moreover, we identified new substrate recognition sites (SRSO, SRS2 SRS3, and 51353.1) and heme binding areas that influence CYP2 structure and function of functional importance as under significant positive selection. Some of the positively selected sites in avian CYP2D are located within the same SRS1 region that was previously linked with the metabolism of plant toxins. Additionally, we find that selective constraint variations in some avian CYP2 subfamilies are consistently associated with different feeding habits (CYP2H and CYP21), habitats (CYP2D, CYP2H, CYP2J, and CYP2K), and migratory behaviors (CYP2D, CYP2H, and CYP2J). Overall, our findings indicate that there has been active enzyme site selection on CYP2 subfamilies and differential selection associated with different life history traits among birds.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17