Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Wegelius, A (Autor) Outra; Khanna, N (Autor) Outra; Esmieu, C (Autor) Outra; Barone, GD (Autor) Outra; Pinto, F (Autor) Outra; Paula Tamagnini (Autor) FCUP; Berggren, G (Autor) Outra; Lindblad, P (Autor) Outra

Revista

Título: Energy and Environmental Sciences

Vol. 11

Páginas: 3163-3167

ISSN: 1754-5692

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 26 Citações

Scopus - 35 Citações

Outras Informações

ID Authenticus: P-00P-WR5

DOI: 10.1039/c8ee01975d

Abstract (EN): [FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H-2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5