NMR Metabolomics Reveals Metabolism-Mediated Protective Effects in Liver (HepG2) Cells Exposed to Subtoxic Levels of Silver Nanoparticles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Carrola, J (Autor) Outra; Pinto, RJB (Autor) Outra; Nasirpour, M (Autor) Outra; Freire, CSR (Autor) Outra; Gil, AM (Autor) Outra; Santos, C (Autor) FCUP; Oliveira, H (Autor) Outra; Duarte, IF (Autor) Outra

Revista

Título: Journal of Proteome Research

Vol. 17

Páginas: 1636-1646

ISSN: 1535-3893

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00N-VRM

DOI: 10.1021/acs.jproteome.7b00905

Abstract (EN): The expansion of biomedical and therapeutic applications of silver nanoparticles (AgNPs) raises the need to further understand their biological effects on human cells. In this work, NMR metabolomics has been applied to reveal the metabolic effects of AgNPs toward human hepatoma (HepG2) cells, which are relevant with respect to nanoparticle accumulation and detoxification. Cellular responses to widely disseminated citrate-coated AgNPs (Cit30) and to emergent biogenic AgNPs prepared using an aqueous plant extract as reducing and stabilizing agent (GS30) have been compared with a view to assess the influence of nanoparticle I coating on the metabolic effects produced. Subtoxic concentrations (IC5 and IC20) of both nanoparticle types caused profound changes in the cellular metabolome, suggesting adaptations in energy production processes (glucose metabolism and the phosphocreatine system), antioxidant defenses, protein degradation and lipid metabolism. These signatures were proposed to reflect mainly metabolism-mediated protective mechanisms and were found to be largely common to Cit30 and GS30 AgNPs, although differences in the magnitude of response, not captured by conventional cytotoxicity assessment, were detected. Overall, this study highlights the value of NMR metabolomics for revealing subtoxic biological effects and helping to understand cell-nanomaterial interactions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11