Somatic embryogenesis of hybrid Pinus elliottii var. elliottii x P-caribaea var. hondurensis and ploidy assessment of somatic plants

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Nunes, S (Autor) Outra; Marum, L (Autor) Outra; Farinha, N (Autor) Outra; Pereira, VT (Autor) Outra; Almeida, T (Autor) Outra; Sousa, D (Autor) Outra; Mano, N (Autor) Outra; Figueiredo, J (Autor) Outra; Dias, MC (Autor) Outra; Santos, C (Autor) FCUP

Revista

Título: Plant Cell, Tissue and Organ Culture

Vol. 132

Páginas: 71-84

ISSN: 0167-6857

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 30 Citações

Outras Informações

ID Authenticus: P-00N-1S2

DOI: 10.1007/s11240-017-1311-7

Abstract (EN): A protocol is described for somatic embryogenesis (SE) Pinus elliottii x P. caribaea, an interspecific pine hybrid of major economic importance. Different embryogenesis conditions were tested, from time of initiation to plant regeneration. Immature megagametophytes containing zygotic embryos of five open pollinated (OP) mother trees were used for SE initiation. An established rate of 10.2% was achieved for explants inoculated on modified Litvay's medium (mLV), supplemented with BAP (4.5 A mu M) and 2,4-D (9 A mu M). Initiation of SE was influenced by the mother-tree genotype however, modification of culture conditions enabled establishment of embryogenic cell lines (ECLs) from all OP seed families. Fifty-two percent of somatic embryos developed to the cotyledonary stage on mLV, supplemented with 40 A mu M ABA and 6% w/v sucrose. Cotyledonary stage somatic embryos converted to plants at 45-86%. Four months after acclimatization in a greenhouse, plant survival rates ranged between 78 and 94%. Flow cytometry analysis of ploidy, did not show any variation in ECLs and somatic seedlings.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14