Comparative analysis between biofilm formation and gene expression in Staphylococcus epidermidis isolates

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Freitas, AI (Autor) Outra; Lopes, N (Autor) Outra; Oliveira, F (Autor) Outra; Bras, S (Autor) Outra; Franca, A (Autor) Outra; Vasconcelos, C (Autor) Outra; Manuel Vilanova (Autor) ICBAS; Cerca, N (Autor) Outra

Revista

Título: Future Microbiology

Vol. 13

Páginas: 415-427

ISSN: 1746-0913

Editora: Future Medicine Ltd.

Indexação

ISI Web of Knowledge - 13 Citações

Scopus - 11 Citações

Outras Informações

ID Authenticus: P-00N-QDF

DOI: 10.2217/fmb-2017-0140

Abstract (EN): Aim: To understand the relationship between ica, aap and bhp gene expression and the implications in biofilm formation in selected clinical and commensal Staphylococcus epidermidis isolates. Material & methods: Isolates were analyzed regarding their biofilm-forming capacity, biochemical matrix composition, biofilm spatial organization and expression of biofilm-related genes. Results: On polysaccharide intercellular adhesin-dependent biofilms, aap and bhp contributions for the biofilm growth were negligible, despite very high levels of expression. In contrast, smaller increases in icaA expression contributed significantly to biofilm growth. Interestingly, no biological differences were observed between clinical and commensal strains. Conclusion: These results reinforce the concept that S. epidermidis is an 'accidental pathogen,' and that the ica operon is the main mechanism of biofilm formation in clinical and commensal isolates.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13