Tetracycline and rifampicin induced a viable but nonculturable state in Staphylococcus epidermidis biofilms

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Carvalhais, V (Autor) Outra; Perez Cabezas, B (Autor) Outra; Oliveira, C (Autor) Outra; Vitorino, R (Autor) FMUP; Manuel Vilanova (Autor) ICBAS; Cerca, N (Autor) Outra

Revista

Título: Future Microbiology

Vol. 13

Páginas: 27-36

ISSN: 1746-0913

Editora: Future Medicine Ltd.

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 15 Citações

Outras Informações

ID Authenticus: P-00N-Q16

DOI: 10.2217/fmb-2017-0107

Abstract (EN): Aim: The goal of this study was to determine the effectiveness of antibiotics on Staphylococcus epidermidis biofilms with different proportions of dormant bacteria, using clinical and commensal isolates. Materials & methods: The ability of S. epidermidis isolates to develop a dormant state was determined. The susceptibility of biofilms with prevented or induced dormancy to antibiotics was evaluated by enumeration of viable and cultivable cells, and confocal microscopy. Results: Dormancy was observed in the majority of tested strains. Tetracycline and rifampicin enhanced the development of a viable but noncultivable biofilm state. Conclusion: Biofilms with induced dormancy were more likely to survive rifampicin. Furthermore, we found that the reduction of cultivable cells was not sufficient to reach definite conclusions on antimicrobial effectiveness.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10