Fast and Reliable Extraction of Polycyclic Aromatic Hydrocarbons from Grilled and Smoked Muscle Foods

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Silva, M (Autor) Outra; Viegas, Olga (Autor) FCNAUP; Melo, A (Autor) Outra; Finteiro, D (Autor) Outra; Pinho, Olívia (Autor) FCNAUP; Ferreira, IMPLVO (Autor) Outra

Revista

Título: Food Analytical Methods

Vol. DOI 10.1007/s12161-012-9387-5

Páginas: 1-10

ISSN: 1936-9751

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 8 Citações

Classificação Científica

CORDIS: Ciências da Saúde

FOS: Ciências médicas e da saúde

Outras Informações

ID Authenticus: P-00P-1AC

DOI: 10.1007/s12161-018-1325-8

Abstract (EN): A fast and simple method for analysis of 14 PAHs in grilled and smoked muscle foods using acetonitrile based-extraction was validated. The optimum amounts of magnesium sulfate and sodium chloride that promoted phase separation of acetonitrile extract containing PAHs from water phase were selected by Central Composite Design. Matrix-matched calibration curves were constructed by adding different concentrations of PAHs and then subjected to extraction followed by HPLC with fluorescent detection. An excellent linearity for all compounds applying weighed least squares linear regression procedure was achieved. LODs and LOQs were lower than 0.12 and 0.39 ng g¿1 respectively. Validation was done according to International Conference on Harmonization recommendations for 14 PAHs. The criteria for 4 EU marker PAHs (benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene) established by European Comission Regulation No. 836/2011 was also accomplished. Repeatability and reproducibility were lower than 8 and 13.3%, and the most of recoveries fall in the range of 80¿110% in different grilled and smoked muscle foods. The proposed method is a robust tool for determination of PAHs in grilled and smoked muscle foods, being easy to perform in short time. © 2018 Springer Science+Business Media, LLC, part of Springer Nature

Idioma: Inglês

Tipo (Avaliação Docente): Científica