Telomerase and N-Cadherin Differential Importance in Adrenocortical Cancers and Adenomas

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Pereira, SS (Autor) ICBAS; Maximo, V (Autor) FMUP; Coelho, R (Autor) Outra; Batista, R (Autor) Outra; Soares, P (Autor) FMUP; Susana Guerreiro (Autor) FCNAUP; Sobrinho Simoes, M (Autor) Outra; Monteiro, M (Autor) ICBAS; D Pignatelli (Autor) FMUP

Revista

Título: Journal of Cellular Biochemistry

Vol. 118 Nº 2

Páginas: 2064-2071

ISSN: 0730-2312

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 5 Citações

Outras Informações

ID Authenticus: P-00N-F9Q

DOI: 10.1002/jcb.25811

Abstract (EN): Adrenocortical carcinomas (ACC) are most frequently highly aggressive tumors. We assessed the telomerase reverse transcriptase (TERT) and N-cadherin role in the biology of ACC and their potential utility as molecular biomarkers, in different types of tumoral adrenocortical tissue. A total of 48 adrenal cortex samples (39 tumoral and 9 normal adrenal glands) were studied. TERT promoter mutations were searched by PCR and Sanger sequencing in two hotspots positions (-124 and -146). Also, telomerase and N-cadherin expression were evaluated by immunohistochemistry. TERT promoter mutations were not detected in any of the samples either malignant or benign. Telomerase nuclear expression was present in 26.6% of ACC and in 45.5% of non-functioning adenomas. It was absent in benign Cushing's lesions and in normal adrenal glands. Contrarily, N-cadherin was always expressed in the cellular membranes of benign adenomas or normal adrenals but no expression was detected in the majority of ACC. Nuclear telomerase and membrane N-cadherin expression were positively correlated in ACCs. We conclude that in ACC, the loss of N-cadherin is a frequent phenomenon while the existence of TERT promoter mutations is not and nuclear telomerase expression is present in only a minority of cases. Since the loss of N-cadherin expression was identified in both high and low proliferative ACC, this marker should be considered important for diagnostic application. Our study also suggests the existence of a TERT non-canonical function in cell adhesion. J. Cell. Biochem. 118: 2064-2071, 2017. (c) 2017 Wiley Periodicals, Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8