Silk fibroin/nanohydroxyapatite hydrogels for promoted bioactivity and osteoblastic proliferation and differentiation of human bone marrow stromal cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Marta Ribeiro (Autor) Outra; Maria H. Fernandes (Autor) FMDUP; Marisa M. Beppu (Autor) Outra; Fernando J. Monteiro (Autor) FEUP; Ferraz, MP (Autor) Outra

Revista

Título: Materials Science and Engineering C

Vol. 89

Páginas: 336-345

ISSN: 0928-4931

Editora: Elsevier

Indexação

ISI Web of Knowledge - 29 Citações

ISI Web of Science

Scopus - 30 Citações

INSPEC

Classificação Científica

CORDIS: Ciências da Saúde

FOS: Ciências médicas e da saúde

Outras Informações

ID Authenticus: P-00Q-4A1

DOI: 10.1016/j.msec.2018.04.034

Abstract (EN): Silk fibroin (SF) is a natural, biocompatible, and biodegradable polymer having a great potential for the successful regeneration of damaged bone tissue. In the present work, nanohydroxyapatite (nanoHA) was incorporated into SF polymer to form a bioactive composite hydrogel for applications as bone implants. The degradation and bioactive properties of SF/nanoHA composite hydrogels were evaluated. Additionally, biological investigations of human bone marrow stromal cells (hBMSCs) viability, proliferation and differentiation to the osteoblastic phenotype were conducted. The incorporation of nanoHA in SF polymer matrices improved the bioactivity of the hydrogels. The biological results highlighted that the SF/nanoHA composite hydrogels are suitable for hBMSCs attachment and proliferation, while a test for alkaline phosphatase (ALP) and bone morphogenetic protein 2 (BMP-2) expression suggested osteoblast differentiation. Additionally, a cell staining method for ALP allowed to observe cell infiltration with active production of ALP by the infiltrated cells, paving the way to use the proposed composite hydrogel for bone tissue regeneration.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10