Structure and function of a novel antioxidant peptide from the skin of tropical frogs

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Barbosa, EA (Autor) Outra; Oliveira, A (Autor) Outra; Placido, A (Autor) Outra; Socodato, R (Autor) Outra; Portugal, CC (Autor) Outra; Mafud, AC (Autor) Outra; Ombredane, AS (Autor) Outra; Moreira, DC (Autor) Outra; Nuno Vale (Autor) ICBAS; Bessa, LJ (Autor) Outra; Joanitti, GA (Autor) Outra; Alves, C (Autor) Outra; Paula Gomes (Autor) FCUP; Delerue Matos, C (Autor) Outra; Mascarenhas, YP (Autor) Outra; Marani, MM (Autor) Outra; Relvas, JB (Autor) ICBAS; Pintado, M (Autor) Outra; Leite, JRSA (Autor) Outra

Revista

Título: Free Radical Biology and Medicine

Vol. 115

Páginas: 68-79

ISSN: 0891-5849

Editora: Elsevier

Indexação

ISI Web of Knowledge - 48 Citações

Scopus - 54 Citações

Outras Informações

ID Authenticus: P-00N-8PG

DOI: 10.1016/j.freeradbiomed.2017.11.001

Abstract (EN): The amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight [M+H](+) = 1543.69 Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12