Determination of Polyamines in Baby Food by Gas Chromatography-Mass Spectrometry: Optimization of Extraction and Microwave-Assisted Derivatization Using Response Surface Methodology

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Petrarca, MH (Autor) Outra; Fernandes, JO (Autor) FFUP; Godoy, HT (Autor) Outra; Cunha, SC (Autor) Outra

Revista

Título: Food Analytical Methods

Vol. 10

Páginas: 3548-3557

ISSN: 1936-9751

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 5 Citações

Outras Informações

ID Authenticus: P-00M-RJ8

DOI: 10.1007/s12161-017-0918-y

Abstract (EN): The present work evaluates the feasibility of microwave-assisted acylation combined with previous ion-pair extraction for the determination of the polyamines spermidine and spermine in baby food by GC-MS. In this way, extraction and derivatization reaction times were simultaneously optimized using a central composite rotational design. From response surface analysis, we verified maximum analytical response for spermidine and spermine employing 1 M hydrochloric acid solution as extraction solvent under shaking during 35 min, followed by acylation derivatization using a household microwave at 600 W for only 5 min. Limits of detection and quantification of 5 and 10 mu g kg(-1) were achieved, and recoveries from 72 to 112% and RSD values ae<currency>16% were obtained under repeatability and within-reproducibility conditions for both polyamines, at levels of 250 and 500 mu g kg(-1). The validated GC-MS method was applied for 20 baby food samples commercially available in Brazil, thus resulting in the first report on spermidine and spermine in baby food.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10