Leishmania infantum Exoproducts Inhibit Human Invariant NKT Cell Expansion and Activation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Belo, R (Autor) Outra; Santarem, N (Autor) Outra; Pereira, C (Autor) Outra; Perez Cabezas, B (Autor) Outra; Macedo, F (Autor) Outra; Leite de Moraes, M (Autor) Outra; Anabela Cordeiro da Silva (Autor) FFUP

Revista

Título: Frontiers In Immunology

Vol. 8

ISSN: 1664-3224

Editora: Frontiers Media

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 7 Citações

Outras Informações

ID Authenticus: P-00N-MET

DOI: 10.3389/fimmu.2017.00710

Abstract (EN): Leishmania infantum is one of the major parasite species associated with visceral leishmaniasis, a severe form of the disease that can become lethal if untreated. This obligate intracellular parasite has developed diverse strategies to escape the host immune response, such as exoproducts (Exo) carrying a wide range of molecules, including parasite virulence factors, which are potentially implicated in early stages of infection. Herein, we report that L. infantum Exo and its two fractions composed of extracellular vesicles (EVs) and vesicle-depleted-exoproducts (VDEs) inhibit human peripheral blood invariant natural killer T (iNKT) cell expansion in response to their specific ligand, the glycolipid alpha-GalactosylCeramide (alpha-GalCer), as well as their capacity to promptly produce IL-4 and IFN gamma. Using plate-bound CD1d and alpha-GalCer, we found that Exo, EV, and VDE fractions reduced iNKT cell activation in a dose-dependent manner, suggesting that they prevented alpha-GalCer presentation by CD1d molecules. This direct effect on CD1d was confirmed by the observation that CD1d: alpha-GalCer complex formation was impaired in the presence of Exo, EV, and VDE fractions. Furthermore, lipid extracts from the three compounds mimicked the inhibition of iNKT cell activation. These lipid components of L. infantum exoproducts, including EV and VDE fractions, might compete for CD1-binding sites, thus blocking iNKT cell activation. Overall, our results provide evidence for a novel strategy through which L. infantum can evade immune responses of mammalian host cells by preventing iNKT lymphocytes from recognizing glycolipids in a TCR-dependent manner.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11