Anandamide interferes with human endometrial stromal-derived cell differentiation: An effect dependent on inhibition of cyclooxygenase-2 expression and prostaglandin E-2 release

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Almada, M (Autor) Outra; Cunha, S (Autor) Outra; Fonseca, BM (Autor) Outra; Amaral, C (Autor) Outra; Piscitelli, F (Autor) Outra; Di Marzo, V (Autor) Outra; Georgina Correia da Silva (Autor) FFUP; Teixeira, NA (Autor) FFUP

Revista

Título: BioFactors

Vol. 42

Páginas: 277-286

ISSN: 0951-6433

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 14 Citações

Scopus - 18 Citações

Outras Informações

ID Authenticus: P-00K-MVD

DOI: 10.1002/biof.1270

Abstract (EN): The human endometrium undergoes cyclical growth, differentiation, and regression periods throughout the reproductive life. The process in which endometrial stromal cells proliferate and differentiate into decidual cells, named decidualization, prepares a receptive endometrium for implantation. Prostaglandins (PGs) and endocannabinoids (eCBs) are crucial mediators of this process. We have recently reported that the eCB anandamide (AEA) interferes with rat stromal cell differentiation, and on the other hand, PGs are also crucial for decidualization. Therefore, in this study, we analyzed the AEA levels, both in nondifferentiated and in decidualizing human endometrial stromal cells by liquid chromatography-mass spectrometry, and investigated the impact of AEA on PG release and cyclooxygenase-2 (COX-2) expression in human endometrial stromal-derived cell differentiation. For that, an ultra-performance liquid chromatography-mass spectrometry/mass spectrometry method to measure prostaglandin E-2 (PGE(2)) and prostaglandin F-2 in biological samples was developed and validated. We demonstrate that AEA levels in decidualizing cells are lower than those in nondifferentiated cells, whereas PGE(2) levels and COX-2 expression are up-regulated. Thus, low AEA levels may be essential for the onset of decidualization. On the contrary, in AEA-treated cells undergoing decidualization, a decrease of COX-2 protein levels and PGE(2) production, in a manner dependent on cannabinoid receptor 1 activation, was observed. Overall, these findings suggest that a deregulation of the intricate network that drives cell differentiation may compromise pregnancy and fertility. It is clinically relevant to understand the mechanisms that influence eCB and PG levels in the endometrium because they may shed light on the sequence of events that lead to a successful pregnancy. (c) 2016 BioFactors, 42(3):277-286, 2016

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10