Evanescent Wave DNA-Aptamer Biosensor based on Long Period Gratings for the Specific Recognition of E. coli

• Tipo: Artigo em Livro de Atas de Conferência Internacional
• Data: 2013

Autores

Queiros, RB (Autor) Outra; Gouveia, C (Autor) Outra; Fernandes, JRA (Autor) Outra; Jorge, PAS (Autor) FCUP

Ata de Conferência Internacional

Título: FIFTH EUROPEAN WORKSHOP ON OPTICAL FIBRE SENSORS

5th European Workshop on Optical Fibre Sensors

Krakow, POLAND, MAY 19-22, 2013

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-006-FTZ

DOI: 10.1117/12.2025813

Abstract (EN): An evanescent wave fiber optic sensor for detection of E. coli outer membranes proteins (EcOMPs) is presented. The sensing probe is achieved by the functionalization of a Long Period Grating (LPG) inscribed in a single mode fiber (SMF28) with poly-L-lysine (PLL) resulting in a label-free configuration capable of specific recognition of EcOMPs in waters due to the resonance wavelength shift variation owing to refractive index changes of the medium (approximate to 100 nm/RIU). The sensing head was characterized and tested against EcOMP and applied to spiked environmental water samples. The sensor displayed linear responses in the range of 1x10(-10) M to 1x10(-8) M EcOMP and is regenerated (under low pH conditions) and the deviation of the subsequent detection was less than 0.1 %.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4