Assessment of ionic liquids' toxicity through the inhibition of acylase I activity on a microflow system

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Azevedo, AMO (Autor) Outra; Pereira, SAP (Autor) Outra; Passos, MLC (Autor) Outra; Costa, SPF (Autor) Outra; Pinto, PCAG (Autor) Outra; Araujo, ARTS (Autor) Outra; Lucia L M F S Saraiva (Autor) FFUP

Revista

Título: Chemosphere

Vol. 173

Páginas: 351-358

ISSN: 0045-6535

Editora: Elsevier

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 9 Citações

Outras Informações

ID Authenticus: P-00M-D9R

DOI: 10.1016/j.chemosphere.2016.12.138

Abstract (EN): Acylase I (ACY I) plays a role in the detoxication and bioactivation of xenobiotics as well in other physiological functions. In this context, an automated ACY I assay for the evaluation of ionic liquids' (Ms) toxicity was developed. The assay was implemented in a sequential injection analysis (SIA) system and was applied to eight commercially available ILs. The SIA methodology was based on the deacetylation of N-acetyl-L-methionine with production of L-methionine, which was determined using fluorescamine. ACY I inhibition in the presence of ILs was monitored by the decrease of fluorescence intensity. The obtained results confirmed the influence of ILs' structural elements on its toxicity and revealed that pyridinium and phosphonium cations, longer alkyl side chains and tetrafluoroborate anion displayed higher toxic effect on enzyme activity. The developed methodology proved to be-robust and exhibited good repeatability (RSD < 1.3%, n = 10), leading also to a reduction of reagents consumption and effluents production. Thus, it is expected that the proposed assay can be used as a novel tool for ILs' toxicity screening.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8