Innate IFN-gamma-Producing Cells Developing in the Absence of IL-2 Receptor Common gamma-Chain

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Resende, M (Autor) Outra; Cardoso, MS (Autor) Outra; Ribeiro, AR (Autor) Outra; Florido, M (Autor) Outra; Margarida Borges (Autor) FFUP; Castro, AG (Autor) Outra; Alves, NL (Autor) Outra; Cooper, AM (Autor) Outra; Appelberg, R (Autor) Outra

Revista

Título: Journal of Immunology

Vol. 199

Páginas: 1429-1439

ISSN: 0022-1767

Editora: American Association of Immunologists

Indexação

ISI Web of Knowledge - 2 Citações

Scopus - 10 Citações

Outras Informações

ID Authenticus: P-00N-H8N

DOI: 10.4049/jimmunol.1601701

Abstract (EN): IFN-gamma is known to be predominantly produced by lymphoid cells such as certain subsets of T cells, NK cells, and other group 1 innate lymphoid cells. In this study, we used IFN-gamma reporter mouse models to search for additional cells capable of secreting this cytokine. We identified a novel and rare population of nonconventional IFN-gamma-producing cells of hematopoietic origin that were characterized by the expression of Thy1.2 and the lack of lymphoid, myeloid, and NK lineage markers. The expression of IFN-g by this population was higher in the liver and lower in the spleen. Furthermore, these cells were present in mice lacking both the Rag2 and the common gamma-chain (gamma c) genes (Rag2(-/-) gamma c(-/-)), indicating their innate nature and their gc cytokine independence. Rag2(-/-) gamma c(-/-) mice are as resistant to Mycobacterium avium as Rag2(-/-) mice, whereas Rag2(-/-) mice lacking IFN-g are more susceptible than either Rag2(-/-) or Rag2(-/-) gamma c(-/-). These lineage-negative CD45(+)/Thy1.2(+) cells are found within the mycobacterially induced granulomatous structure in the livers of infected Rag2(-/-) gamma c(-/-) animals and are adjacent to macrophages that expressed inducible NO synthase, suggesting a potential protective role for these IFN-gamma-producing cells. Accordingly, Thy1.2specific mAb administration to infected Rag2(-/-) gamma c(-/-) animals increased M. avium growth in the liver. Overall, our results demonstrate that a population of Thy1.2(+) non-NK innate-like cells present in the liver expresses IFN-g and can confer protection against M. avium infection in immunocompromised mice.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11