In vitro multimodal-effect of Trichilia catigua A. Juss. (Meliaceae) bark aqueous extract in CNS targets

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Bernardo, J (Autor) Outra; Ferreres, F (Autor) Outra; Gil Izquierdo, A (Autor) Outra; Videira, RA (Autor) Outra; Patricia Valentao (Autor) FFUP; Veiga, F (Autor) Outra; Paula B Andrade (Autor) FFUP

Revista

Título: Journal of Ethnopharmacology

Vol. 211

Páginas: 247-255

ISSN: 0378-8741

Editora: Elsevier

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 13 Citações

Outras Informações

ID Authenticus: P-00N-3A2

DOI: 10.1016/j.jep.2017.09.039

Abstract (EN): Ethnopharmacological relevance: The bark of Trichilia catigua A. Juss. (Meliaceae), popularly known as "big catuaba", is traditionally used in Brazilian folk medicine for its neuroactive potential as memory stimulant, and antinociceptive and antidepressant effects. Aim of the study: To study the aqueous extract of T. catigua bark as dual inhibitor of monoamine oxidase A (MAO-A) and acetylcholinesterase (AChE). To explore its antioxidant potential through interaction with xanthine/xanthine oxidase (X/XO) pathway, and to attempt a relationship between its phenolic profile and effects displayed. Materials and methods: Phenolic profiling was achieved by HPLC-DAD-ESI/MSn and UPLC-ESI-QTOF-MS analyses. The capacity to inhibit hMAO-A was assessed in vitro, as was that for AChE, evaluated in rat brain homogenates. The direct inhibition of the X/XO pathway and the scavenging of superoxide anion radical were the selected in vitro models to explore the antioxidant potential. The cytotoxic effects were assayed in the human neuronal SH-SY5Y cells by MTT reduction, after direct exposure (24 h). Results: Twenty-six compounds were identified and quantified (551.02 +/- 37.61 mg/g of lyophilized extract). The phenylpropanoid substituted flavan-3-ols were the most representative compounds (similar to 81% of quantified mass). The extract inhibited hMAO activity in a concentration-dependent manner (IC50 = 121.06 +/- 2.13 mu g/mL). A mixed model of inhibition of AChE activity was observed, reflected by the pronounced increase of Km values and a more discreet effect over the Vmax parameters, calculated from Michaelis-Menten fitted equations. In addition, it was demonstrated that the extract directly inhibits the X/XO pathway (IC50 = 121.06 +/- 2.13 mu g/mL) and also imbalances the oxidative stress acting as superoxide anion radical scavenger (EC50 = 104.42 +/- 10.67 mu g/mL), an oxidative by-product of this reaction. All these neuroprotective and neurotrophic effects were displayed within the non-toxic range of concentrations (0.063-0.500 mu g/mL) in SH-SY5Y cells. Conclusions: Our results validate the traditional use of T. catigua bark for its neuroactive and neuroprotective potential. A novel approach upon its application towards the management of neurodegenerative and related symptomatology was likewise demonstrated.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9