Rolipram promotes functional recovery after contusive thoracic spinal cord injury in rats

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Costa, LM (Autor) Outra; Pereira, JE (Autor) Outra; Filipe, VM (Autor) Outra; Magalhaes, LG (Autor) Outra; Couto, PA (Autor) Outra; Gonzalo Orden, JM (Autor) Outra; Raimondo, S (Autor) Outra; Geuna, S (Autor) Outra; Mauricio, AC (Autor) ICBAS; Nikulina, E (Autor) Outra; Filbin, MT (Autor) Outra; Varejao, ASP (Autor) Outra

Revista

Título: Behavioural Brain Research

Vol. 243

Páginas: 66-73

ISSN: 0166-4328

Editora: Elsevier

Indexação

ISI Web of Knowledge - 31 Citações

Scopus - 32 Citações

Outras Informações

ID Authenticus: P-005-1JS

DOI: 10.1016/j.bbr.2012.12.056

Abstract (EN): Numerous animal model studies in the past decade have demonstrated that pharmacological elevation of cyclic AMP (cAMP) alone, or in combination with other treatments, can promote axonal regeneration after spinal cord injury. Elevation of cAMP via the phosphodiesterase 4 (PDE4) inhibitor, rolipram, decreases neuronal sensitivity to myelin inhibitors, increases growth potential and is neuroprotective. Rolipram's ability to cross the blood-brain barrier makes it a practical and promising treatment for CNS regeneration. However, several studies have questioned the efficacy of rolipram when given alone. The purpose of this investigation was to determine the effects of continuous administration of rolipram, given alone for 2 weeks, following a moderate T10 contusion injury in rat. Functional recovery was evaluated using the 21-point Basso, Beattie and Bresnahan (BBB) locomotor recovery scale and the beam walk. We used threedimensional (3D) instrumented gait analysis to allow detailed assessment and quantification of hindlimb motion. The amount of the damaged tissue and spared white matter was estimated stereologically. Our results show that administration of rolipram following acute spinal cord contusion results in improved motor performance at each time-point. Dynamic assessment of foot motion during treadmill walking revealed a significantly decreased external rotation during the entire step cycle after 8 weeks in rolipram-treated animals. Stereological analysis revealed no significant differences in lesion volume and length. By contrast, spared white matter was significantly higher in the group treated with rolipram. Our results suggest a therapeutic role for rolipram delivered alone following acute SCI.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8